ABSTRACT
Since the discovery of plasmid, various methods have been developed to isolate plasmid DNA. All the methods have one common and important target of isolating plasmid DNA of high quality and quantity in less time. These methods are not completely safe because of use of toxic chemicals compounds. The developed protocol for plasmid extraction is based on the alkaline lysis method of plasmid preparation (extraction at pH 8.0) with slight modifications. Cell lysis reagent sodium dodecyl sulfate is replaced by lipase enzyme present in laundry detergent. A good plasmid preparation can be made, which is well suited for subsequent molecular biology applications. By taking safety measures on count, contaminants like, RNA and protein can be completely avoided with maximized plasmid yield. The resultant plasmid quality and quantity can be well comparable to other prevalent methods.
ABSTRACT
A hospital based interdepartmental collaborative study was carried out from 1st July to 30th September, 2000 on 269 rural people residing in six districts of the northern part of West Bengal and attending the Pathology Department in North Bengal Medical College for Fine Needle Aspiration Cytology (FNAC) after being referred from different clinical departments. The objectives of the study were to study some aspects of the outcomes of FNAC among rural people and to suggest for its wider application in rural community through Community Health Centre/Rural Hospital/Block Primary Health Centre. The results shows that Hindus (80.3%) are attending more in number than Muslims (14.13%), Christians (4.83%) and Buddhist (0.74%); 53.54% of the population are in 11-40 years age group. In benign conditions 76.95% are coming from up to 100 kms. of N.B.M.C; people in lower per capita income group of up to Rs.400/- per month are attending twice in number than those in the income groups of Rs.401/- and more per month. In malignancy however people do not think of distance or expenses due to seriousness of the diseases. In 14.5% cases FNAC remains inconclusive whereas in 85.5% cases it provides definite diagnoses.
Subject(s)
Adolescent , Adult , Aged , Biopsy, Needle/statistics & numerical data , Child , Child, Preschool , Female , Health Services Research , Hospitals, Rural , Humans , India , Infant , Male , Middle Aged , Neoplasms/diagnosis , Patient Acceptance of Health Care/ethnology , Religion , Rural Population , Schools, MedicalABSTRACT
Glanzmann's thrombasthenia is a well defined inherited disorder of platelet function characterized by qualitative and qualitative defect in cytoadhesive membrane protein, glycoprotein IIb-IIIa (the platelet fibrinogen receptor). From January 1990 to October, 1999, five patients who presented with mucocutaneous bleeding were detected to have Glanzmann's thrombasthenia. Clinical and laboratory spectrum of this rare disorder was studied which revealed heterogeneity of disease with respect to nature and severity of bleeding unpredictable by laboratory findings.
Subject(s)
Adolescent , Blood Platelets/pathology , Child , Child, Preschool , Female , Hemorrhage , Hemostasis , Humans , Male , Platelet Aggregation , Platelet Glycoprotein GPIIb-IIIa Complex/metabolism , Thrombasthenia/physiopathologyABSTRACT
OBJECTIVE: To determine the creamatocrit values of breastmilk at different lactational periods during the first 6 months after delivery and its relation to infant growth. DESIGN: Prospective follow-up study. SETTING: Maternity and well baby clinics of a metropolitan teaching, a rural teaching and a suburban municipal hospital. SUBJECTS: 1322 exclusively breastfed infants and their mothers. There were 160 preterm, 281 term low birth weight (LBW) and 881 term appropriate for date (AFD) babies. METHODS: Creamatocrit of breastmilk and infant's body weight were recorded at 4,8,12,16,20 and 24 weeks after birth. RESULTS: Creamatocrit values of breastmilk in all mothers increased upto 16 post partum weeks and then declined. There were no significant differences in creamatocrit values of breastmilk of mothers of preterm, term LBW and term non-LBW infants. Preterm and term LBW infants showed a catch up in body weight with the term AFD group by 20 weeks post-partum. CONCLUSIONS: The breastmilk creamatocrit progressively rises upto the fourth month of lactation but is not influenced by gestation or intra uterine growth retardation. There is no relationship between breastmilk creamatocrit and infant growth. Exclusive breast feeding adequately supports growth of LBW infants in comparison to their normal peers during the first 6 months of life.
Subject(s)
Body Weight , Breast Feeding , Female , Gestational Age , Humans , Infant , Infant, Low Birth Weight , Infant, Newborn , Milk, Human/chemistry , Prospective StudiesABSTRACT
A trypsin like serine-proteinase of M(r) 16,000 Da, optimally active at pH 8.4 on N-benzoyl-arginine ethyl ester (BAEE) was purified from 4-day old germinated seeds of rice bean, Vigna umbellata (Thunb), by ammonium sulphate precipitation, gel filtration, ion-exchange chromatography and by high performance liquid chromatography (HPLC). The purity of the enzyme was checked by polyacrylamide gel electrophoresis (PAGE). The enzyme activity was studied on natural substrates like casein, haemoglobin and vicilin, a rice bean storage protein. The activity of the enzyme was completely inhibited by phenylmethylsulfonyl fluoride, but not by iodoacetamide and HgCl2, suggesting it to be a serine protease. Loss of activity in presence of EDTA was reversed by addition of Ca2+.